Sequence Assembly Lecture
Overview
Teaching: 30 min
Exercises: 0 minQuestions
How can the information in the sequencing reads be reduced?
What are the different methods for assembly?
How can we assess the quality of an assembly?
Objectives
Understand differences between assembly methods
Understanding different assembly quality analysis methods
Sequence assembly and assembly quality
Next up is a lecture which details assembly strategies, different kind of assemblers and ways of assessing assembly quality. If you have any questions please raise your hand. You should be able to know the difference between the contigs and the scaffolds and how to assess the quality of an assembly.
The slides are available here: files/MicrobialGenomics_AssemblyStrategies_Linda_Triumph.pptx
Alternatively, you can follow the lecture by Professor Bas Dutilh if you take this course on your own. This lecture details assembly strategies, different kind of assemblers and ways of assessing assembly quality and binning of contigs when doing metagenomic assembly.
The lecture can be found here: https://www.youtube.com/watch?v=mHmMbPxKmn0 Slide handouts of this extensive lecture are available at https://klif.uu.nl/klif/mgen/MicrobialGenomics_AssemblyStrategies_Linda.pdf
This lecture takes 60 minutes.
Key Points
Assembly is a process which aligns and merges fragments from a longer DNA sequence in order to reconstruct the original sequence.
Quality can be assessed using N50 but also using other methods